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Abstract: PO0201

Serum Proteomics Identifies Protein Alterations After Platinum-Based Chemotherapy

Session Information

Category: Acute Kidney Injury

  • 101 AKI: Epidemiology, Risk Factors, and Prevention

Authors

  • Li, Shensen, Sir Run Run Hospital Nanjing Medical University, Nanjing, Jiangsu, China
  • Liu, Qingqing, Sir Run Run Hospital Nanjing Medical University, Nanjing, Jiangsu, China
  • Shen, Haiyan, Sir Run Run Hospital Nanjing Medical University, Nanjing, Jiangsu, China
  • Li, Wenwen, Sir Run Run Hospital Nanjing Medical University, Nanjing, Jiangsu, China
  • Cao, Changchun, Sir Run Run Hospital Nanjing Medical University, Nanjing, Jiangsu, China
Background

Acute kidney injury (AKI) is a common complication after platinum-based chemotherapy. However, the mechanisms mediating this process remain to be fully understood. Our object was to invest serum proteins variations among patients after receiving platinum-based chemotherapy to provide more evidence of the pathophysiology of chemotherapy induced AKI.

Methods

In this study, serum samples from 10 patients underwent chemotherapy were used and samples before therapy were used as control. Serum proteins were extracted and submitted for LC-MS/MS. Peptides were analyzed for spectral count quantitation. The classification and enrichment analysis of these factors were based on Gene Ontology and Kyoto Encyclopedia of Genes and Genomes.

Results

An overview of GO enrichment showed that organization biogenesis, response to stimulus and immune system process were significantly changed after chemotherapy. Protein binding and transporter activity were the main differences of molecular function in the GO assignments. KEGG analysis revealed that several pathways were highly enriched including infectious disease, immune system, transport and catabolism. After narrowing down the targets and compared with the control status, top 5 significantly up-regulated proteins were HBD, HBB, HBG2, HBA1, TNC and top 5 down-regulated proteins ADAMTS13, FLT4, HRNR, MINPP1 and KRT9. These proteins were mostly involved in immune regulation, cellular component and organization biogenesis.

Conclusion

The serum proteomic is distinct after platinum-based chemotherapy. Inflammation and extracellular related proteins were involved in renal disease.

Funding

  • Government Support – Non-U.S.