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Abstract: SA-PO751

The Differential Expression Research of Circular RNAs in Exosomes from Serum and Urine in Patients with Idiopathic Membranous Nephropathy

Session Information

  • CKD: Mechanisms - III
    November 09, 2019 | Location: Exhibit Hall, Walter E. Washington Convention Center
    Abstract Time: 10:00 AM - 12:00 PM

Category: CKD (Non-Dialysis)

  • 2103 CKD (Non-Dialysis): Mechanisms

Author

  • Ma, Hualin, Shenzhen People's Hospital, Shenzhen, GUANGDONG, China
Background

To further explore the pathogenesis of IMN,the technique of gene-sequencing was uesd to analyze the differentially expressed circRNAs in exosomes from both the serum and urine of patients with IMN,which may lay the foundation for research of circRNAs as a new class of exosome-base IMN diagnosis biomarkers.

Methods

Ten patients with IMN and ten normal controls were recruited as experimental subjects in our study. The exosomes were extracted from the collected serum and urine. Then, pure circRNAs were extracted from the exosomes with a series of enzymatic reactions. Afterwards, the significantly differentially expressed circRNAs were chosen by the method of gene-sequencing.

Results

Compared with normal controls. the circRNAs were reduced in the exosomes from serum of patients with IMN, which mostly originated from intron gene regions. Meanwhile, a total of 89 circRNAs were significantly differentially expressed, which were also mostly derived from intron gene regions. including 49 up-regulated and 40 down-regulated genes. However , the species were increased in the exosomes from the urine of patients with IMN compared to normal controls, and they mainly originated from exon gene regions. Simultaneously, a total of 60 circRNAs were significantly differentially expressed, which primarily belonged to intron gengeregions, including 54 up-regulated and 6 down-regulated regions.

Conclusion

The significant differential and specific expression of circRNAs in the exosomes from patients with IMN were. For example, MUCA, which originated from chr7:100550808/100551062, could be considered a potential diagnostic biomarker of IMN. Furthermore, these figures may be used as a reference or supplement in the research of the pathogenesis of IMN.
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