Abstract: SA-PO634
Biofluid MicroRNA Expression Patterns in Three Types of Naturally Occurring Canine Models for Glomerular Disease
Session Information
- Glomerular Diseases: ANCA, Anti-GBM, Kidney Biopsy
November 09, 2019 | Location: Exhibit Hall, Walter E. Washington Convention Center
Abstract Time: 10:00 AM - 12:00 PM
Category: Glomerular Diseases
- 1203 Glomerular Diseases: Clinical, Outcomes, and Trials
Authors
- Chu, Candice Pei-Hua, Texas A&M University, College Station, Texas, United States
- Cianciolo, Rachel, The Ohio State University, Columbus, Ohio, United States
- Hokamp, Jessica, The Ohio State University, Columbus, Ohio, United States
- Lees, George E., Retired, Lakeland, Florida, United States
- Nabity, Mary B., Texas A&M University, College Station, Texas, United States
Background
The majority of proteinuric dogs with naturally-occurring chronic kidney disease (CKD) have one of three categories of glomerular diseases: immune complex-mediated (often membranous glomerulopathy [MGN]), glomerulosclerosis (GS), or amyloidosis (AMYL). As in humans, proper treatment of glomerular disease relies on an accurate diagnosis largely based on a renal biopsy and comprehensive pathologic examination. We hypothesized that the expression pattern of biofluid microRNA (miRNAs) would correlate with disease progression and categorization.
Methods
Archived serum and urine samples from 24 dogs, 6 proteinuric dogs from each glomerular disease category (MGN, GS, and AMYL) and 6 clinically healthy dogs were selected. Within each glomerular disease category, equal numbers of non-azotemic and azotemic dogs were included. Circulating and urinary miRNAs were isolated and profiled using small RNA sequencing.
Results
Overall, 38 circulating miRNAs and 16 urinary miRNAs were differentially expressed (DE) in CKD dogs versus controls. When all CKD dogs were combined regardless of glomerular disease category, no circulating DE miRs were identified between azotemic and non-azotemic CKD dogs. However, DE urinary miR-182, miR-21, and miR-486 were identified comparing azotemic dogs versus non-azotemic CKD dogs. Notably, the distinctive expression of urinary miR-126, miR-335, and miR-128 could correctly group azotemic, proteinuric dogs into MGN, GS, or AMYL.
Conclusion
This unique finding supports that urinary miRNAs might help establish a diagnosis in azotemic dogs with suspected glomerular disease. These highly conserved miRNAs are potential non-invasive biomarkers for human patients.
Differentially expressed miRNAs in proteinuric dogs with three types of glomerular disease
Funding
- Private Foundation Support