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Abstract: SA-PO826

The Effect of Bilateral Nephrectomy on Gene Expression of Hypothalamic Neuropeptides Regulating Feeding Behaviors

Session Information

Category: Health Maintenance, Nutrition, and Metabolism

  • 1300 Health Maintenance, Nutrition, and Metabolism

Authors

  • Nakazono, Kazutoshi, University of Occupational and Environmental Health,Japan, Kitakyuusyuu-shi, Japan
  • Ueno, Hiromichi, University of Occupational and Environmental Health, Kitakyushu, Japan
  • Sanada, Kenya, University of Occupational and Environmental Health, Japan, Kitakyushu-shi, Japan
  • Fujimoto, Yoko, University of Occupational and Environmental Health, Kitakyushu, Japan
  • Ueta, Yoichi, University of Occupational and Environmental Health, Kitakyushu, Japan
  • Otsuji, Yutaka, University of Occupational and Environmental Health, Kitakyushu, Japan
  • Miyamoto, Tetsu, Second Department of Internal Medicine, Kitakyushu, Fukuoka, Japan
Background

Anorexia is one of the most widespread eating disorders that appears to contribute to malnutrition in patients with advanced kidney dysfunction. Previously, many neuropeptides synthesized in the hypothalamus have been shown to regulate feeding behavior. While several mechanisms underlying uremic anorexia have been proposed, the hypothalamic neuropeptides that regulate feeding in the hypothalamus of patients with kidney dysfunction are poorly understood.

Methods

The gene expressions of hypothalamic neuropeptides controlling feeding behaviors were evaluated after bilateral nephrectomy, which is a model of acute kidney dysfunction, by in situ hybridization histochemistry. Adult male rats received bilateral nephrectomy or a sham operation under anesthesia. The rats were decapitated at 6, 12, and 24 h after treatment. The gene expression of corticotrophin-releasing hormone (CRH) in the paraventricular nucleus (PVN); proopiomelanocortin (POMC), cocaine- and amphetamine-regulated transcript (CART), neuropeptide Y (NPY), agouti-related peptide (AgRP) in the arcuate nucleus; and melanin-concentrating hormone (MCH) and orexin in the lateral hypothalamic area, were quantified by in situ hybridization histochemistry. After treatment, cumulative food intake, water intake, and body weight were measured.

Results

Food consumption decreased markedly in bilateral nephrectomized rats. The mRNA levels of CRH, POMC, CART, which suppress feeding behavior, were significantly higher in bilateral nephrectomized rats than in sham-operated rats. On the other hand, the mRNA levels of NPY, AgRP, MCH, and orexin, which promote feeding behavior, were significantly lower in bilateral nephrectomized rats than in sham-operated rats.

Conclusion

The results suggest that hypothalamic neuropeptides regulating feeding behaviors may be involved in the development of anorexia in bilateral nephrectomized rats. This report is the first step to elucidating the physiological mechanisms of anorexia in patients with kidney dysfunction.