Abstract: TH-PO079
Renal Events Following Iodinated Contrast Aggravate Diabetic Nephropathy
Session Information
- AKI: Epidemiology, Risk Factors, Prevention - I
November 07, 2019 | Location: Exhibit Hall, Walter E. Washington Convention Center
Abstract Time: 10:00 AM - 12:00 PM
Category: Acute Kidney Injury
- 101 AKI: Epidemiology, Risk Factors, and Prevention
Authors
- Fernandes, Sheila Marques, University of São Paulo, Carapicuíba, Brazil
- Brandi, Beatriz De almeida, Universidade de São Paulo, São Paulo, Brazil
- Peres, Karina Batista, EEUSP, São Paulo, Brazil
- Santos, Luciana soares Costa, Universidade de São Paulo, São Paulo, Brazil
- da Fonseca, Cassiane Dezoti, Federal University of Sao Paulo, Sao Paulo, SP, Brazil
- Watanabe, Miriam M., Faculdades Metrpolitana Unidas, São Paulo, Brazil
- Vattimo, Maria De Fatima, Universidade de São Paulo, São Paulo, Brazil
Group or Team Name
- Group of Studies on Acute Kidney Injury (GERA)
Background
Iodinated contrast (IC) is a leading cause of AKI (CI-AKI) and occurs more frequently in individuals with increasingly common risk factors, including diabetes. IC-AKI has been growing in recent years faced with the more frequent diagnostic needs in old patients and with comorbid conditions such as diabetes. This complication is due to a number of factors, including the osmolality of the IC.
The aim of this study is to evaluate the injury in kidneys of diabetic rats submitted to treatment with high- osmolar and low-osmolar iodinated contrast, evaluating the impact on hemodynamic and renal function in addition to oxidative profile.
Methods
Wistar rats, male and adult, weighing 250-290g were randomized into four groups: Citrate, Diabetes Mellitus (DM), Diabetes+low-osmolar iodinated contrast (DM+LIC) and DM+high-osmolar iodinated contrast (DM+HIC). Physiological parameters (body weight, water and food intake, glycemia and kidney/body weight ratio); renal function (Inulin Clearance; urinary neutrophil gelatinase/uNGAL); hemodynamics (arterial blood pressure; renal blood flow/RBF; renal vascular resistance/RVR) and oxidative profile (urinary peroxides/UP, urinary TBARS, renal tissue thiols and urinary nitric oxide/NO) were evaluated.
Results
Diabetic groups showed polyphagia, polydipsia, polyuria, high levels of blood glucose and reduction in body weight. DM group showed a reduced inulin clearance, elevated uNGAL, elevated RVR, reduced RBF, elevated UP, TBARS and NO and a consumption of antioxidant reserve. When IC was introduced, the parameters of renal function, renal hemodynamic and oxidative profile became worse, specially in the group DM+HIC.
Conclusion
The use of high and low osmolarity IC promoted additional deleterious action to renal function and hemodynamics with oxidative injury in diabetic rats, with a more expressive effect in the group submitted to high osmolality contrast treatment.
Renal function, hemodynamics, oxidative
| Group (n) | Serum creatinine (mg/min/kg) | Inulin clearance (mL/min/kg) | Neutrophil gelatinase-associated lipocalin (ng/mL) | Renal blood flow (mL/min) | Renal vascular resistance (mmHg/mL/min) | Urinary peroxides (nmol/g creatinine) | TBARS (nmol/g creatinine) | Thiols (nmol/mg potein) | Nitric oxide (µM/g creatinine) |
| Citrate (7) | 0.30±0,06 | 0.94±0.23 | 41.41±4.63 | 8.19±0.76 | 15.05±2.00 | 2.15±0.85 | 0.16±0.04 | 30.92±7.44 | 25.06±4.05 |
| DM (7) | 0.80±0,25 * | 0.58±0.05 * | 57.25±14.44 | 3.96±0.70* | 26.34±5.60 * | 12.74±3.94 * | 1.09±0.28* | 15.75±1.17 * | 53.72±5.31 * |
| DM+LIC | 0.87±0,11 *# | 0.34±0.11 *# | 103.78±9.09 *# | 3.06±0.42 *# | 35.42±6.35 *# | 18.94±7.54 *# | 2.13±0.46 *# | 12.50±3.64 *# | 76.78±8.47 *# |
| DM+HIC | 1.47±0,66 *#& | 0.14±0.01 *#& | 167.27±8.50 *#& | 2.29±0.55 *#& | 44.97±6.06 *#& | 25.57±7.54 *#& | 3.31±0.47 *#& | 6.22±0.31 *#& | 88.23±8.47 *& |
* p<0.05 versus Citrate; # p<0.05 versus DM; & p<0.05 versus DM+LIC
Funding
- Government Support - Non-U.S.