Abstract: SA-PO747
Omega-3 Polyunsaturated Fatty Acids Reduce Intestinal Inflammation and Enhance Intestinal Motility Associated with Reduced Nitric Oxide Production in CKD
Session Information
- CKD: Mechanisms - III
November 09, 2019 | Location: Exhibit Hall, Walter E. Washington Convention Center
Abstract Time: 10:00 AM - 12:00 PM
Category: CKD (Non-Dialysis)
- 2103 CKD (Non-Dialysis): Mechanisms
Authors
- Yu, Chao, Department of Nephrology, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China
- Liu, Feng, Shanghai East Hospital, Shanghai, China
- Zhuang, Shougang, Department of Nephrology, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China
Background
Previous studies have shown that chronic kidney disease (CKD) could elicit an intestinal inflammation and result in intestinal dysmotility followed by many complications. Increasing evidence suggests that omega-3 polyunsaturated fatty acids can reduce intestinal inflammation and improve intestinal function in many diseases. In this study, we therefore investigated the effect of omega-3 polyunsaturated fatty acids on intestinal inflammation and intestinal motility in CKD and the underlying mechanism.
Methods
CKD was induced by the 5/6 kidney resection, and omega-3 polyunsaturated fatty acids enriched diet or standard diet was administrated for six weeks. Intestinal motility was assessed by charcoal transportassay, and intestinal inflammation was assessed by analyzing myeloperoxidase activity and concentrations and gene expression of TNF-α,IL-1β, and IL-10 in the intestinal tissue. The nitric oxide production was assessed in the intestinal tissue.
Results
The results showed that CKD resulted in a marked delay in intestinal motilityand was associated with a significant increase of intestinal levels of inflammatory parameters (P<0.05). However, compared to the standard diet,omega-3 polyunsaturated fatty acids enriched diet administration markedly reduced intestinal inflammatory response, and resulted in a significant improvement in intestinal motility(P<0.05). In addition, the intestinal nitric oxide production was inhibited by omega-3 polyunsaturated fatty acids enriched diet treatment (P<0.05).
Conclusion
These results suggest that omega-3 polyunsaturated fatty acids could reduce intestinal inflammation and enhance intestinal motility in CKD, and the underlying mechanism may be associated with reduced nitric oxide production.
Funding
- Government Support - Non-U.S.