Abstract: SA-PO949
Pasp Deletion Mutation Reduced the Virulence and Pathogenic Ability of Pseudomonas aeruginosa
Session Information
- Peritoneal Dialysis: Inflammation, Peritoneal Transport
November 09, 2019 | Location: Exhibit Hall, Walter E. Washington Convention Center
Abstract Time: 10:00 AM - 12:00 PM
Category: Dialysis
- 703 Dialysis: Peritoneal Dialysis
Authors
- Guan, Tianjun, xiamen zhongshan hospital, Xiamen, Fujian, China
- Chen, Anqun, ZhongShan Hospital, Xiamen University, Xiamen, China
- Ziming, Wan, The first affiliated hospital of chongqing medical university, Chongqing, Chongqing, China
Background
Pseudomonas aeruginosa is a very important conditional pathogen commonly existing in the environment, and it is the main pathogen of catheter-related infection in peritoneal dialysis. The formation of intraductal biofilm is an important reason, and the expression of PasP protein is increased in Pseudomonas aeruginosa, which is easy to form biofilm. It is intended to further explore the effect of PasP protein on the virulence of Pseudomonas aeruginosa.
Methods
the pasP gene of Pseudomonas aeruginosa PAO1 was knocked out by homologous recombination method, and the PAO1::pasP strain was constructed. The following parameters of parental bacteria and homologous recombinant bacteria were observed: colony morphology, biofilm forming ability, minimum bactericidal concentration of common antibiotics to bacteria, tolerance of bacteria to temperature, determination of bacterial virulence (pyocyanin, protease, elastase). Difference analysis of protein spectrum were performed;
Results
the pasP gene of Pseudomonas aeruginosa PAO1 was successfully deleted, PAO1::pasP was successfully constructed by homologous recombination. The relative virulence of PAO1::pasP was weaker than that of parent bacteria, including the weakening of biofilm formation ability and the decreasing tolerance to temperature. the production of pyocyanin, protease, elastase were all decreased. The expressions of two-component reaction regulator, two-component sensor, ATP binding protein of ABC transporter, bacteriophage protein, purine binding protein, and the two-component reaction regulator, two-component sensor, ATP binding protein, bacteriophage protein and purine binding protein of PTX transporter decreased, Aer2 protein, type IV flagellin Flp and rhamnose glycosyltransferase subunit A were significantly lower than in PAO1::pasP strain than in parental bacteria.
Conclusion
Pseudomonas aeruginosa pasP gene is involved in the biofilm formation and QS sensing system, and pasP gene deficient strains show weaker bacterial virulence and pathogenic ability.