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Kidney Week

Abstract: FR-PO1114

Non-Cultured Adipose-Derived Regenerative Cells Limit Early Inflammation and Fibrosis in Renal Ischemia-Reperfusion Injury

Session Information

  • Transplantation: Basic
    November 08, 2019 | Location: Exhibit Hall, Walter E. Washington Convention Center
    Abstract Time: 10:00 AM - 12:00 PM

Category: Transplantation

  • 1901 Transplantation: Basic


  • Lathan, Rashida, University of Glasgow, Glasgow, United Kingdom
  • Mark, Patrick B., University of Glasgow, Glasgow, United Kingdom

Studies in our rat model of ischemic reperfusion injury (IRI) demonstrate improved kidney function post injection of adipose-derived regenerative cells (ADRC). The mechanism on how these cells induce reparative effects during IRI remains elusive. We investigated ADRC-derived effects within the injured kidney at early timepoints.


Inguinal rat ADRC or vehicle control were injected via the renal artery of the IRI rat model. At 48 hours and 1-week post-ischemia injury, kidney was evaluated for fibrotic and inflammatory markers through qPCR and western blotting of (n=6-8). Leukocyte quantity was assessed by flow cytometry (n=4-5). Histology was used to measure infiltrative lesions and Masson Trichrome stained collagen accumulation (n=8-20).


ADRC-treated kidneys expressed lower levels of inflammatory gene CXCL12 and significantly lower protein levels of granulocyte macrophage colony-stimulating factor (both p<0.05). In addition, a consistent increase in cytotoxic T-lymphocyte-associated protein 4 transcript was characteristic of ADRC treated kidneys. At 48 hours post-IRI, half of vehicle controls contained higher levels of CD45+ leukocytes. Assessment of leukocyte infiltrate indicated a trend of higher infiltrate in vehicle control kidneys compared to ADRC kidneys at 48 hours with significant apparent differences by 1-week post IRI (p<0.05).
Early accumulation of interstitial factors: tissue inhibitor of metalloproteinase-1 (p<0.05) and collagen type 1, alpha 2 (p<0.001) in vehicle control kidneys indicated early fibrotic development. This was mirrored by significantly high levels of collagen staining in control compared to ADRC treated kidneys at 1-week post IRI (p<0.001).


Collectively, gene, protein expression and histological evidence suggest that ADRC treated IRI kidneys experience early anti-inflammatory changes conducive to the inhibition of fibrogenesis.


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