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Abstract: FR-PO829

CD4+T Cell Abnormalities in the Myeloperoxidase-ANCA Associated Vasculitis

Session Information

Category: Glomerular Diseases

  • 1202 Glomerular Diseases: Immunology and Inflammation

Authors

  • Liao, Zhonghua, Xiangya Hospital, Central South University, Changsha, Hunan, China
  • Tang, Jiale, Xiangya Hospital, Central South University, Changsha, Hunan, China
  • Hu, Xinyue, Xiangya Hospital, Central South University, Changsha, Hunan, China
  • Deng, Shuanglinzi, Xiangya Hospital, Central South University, Changsha, Hunan, China
  • Feng, Juntao, Xiangya Hospital of Central South University, Changsha, Hunan, China
  • Li, Xiaozhao, Xiangya Hospital, Central South University, Changsha, Hunan, China
Background

In ANCA-associated vasculitis ,MPO-specific CD4 +T cells have been reported to involve in renal injury. Although in Granulomatosis with Polyangiitis (GPA), it has been found that lymphopenia is a common clinical feature, and the effect of treatment was excluded. However the pathogenesis of T cell recruitment is not fully elucidated. Thus, we dissect whether the microscopic polyangiitis patients have the similar symptoms and the underlying pathogenesis.

Methods

Clinical data from 143 newly diagnosed microscopic polyangiitis’ patients (Collection time prior to use of Glucocorticoid and immunosuppressants) and 176 healthy controls was collected and analyzed. The phenotypic characterization of peripheral blood lymphocyte in 33 of MPA patients was measured by Flow cytometry. Meanwhile, disease activity of these patients according to the Birmingham Vasculitis Activity Score was marked. The cytokine produced by relevant CD4+T cells was detected. Chemokine and chemokine receptors axis concerning T cell recruitment was observed by immunohistochemistry in formaldehyde-fixed kidney nephridial tissue.

Results

MPA patients’ lymphocyte count existing in peripheral blood was significantly decreased compared with healthy control. Besides, abnormal CD4+T cell subsets was found in MPA patients . Specifically , the proportion of Th1, Th9, Th17,Th22 was increased in these patients (BVAS≥15 points). In accordance with this ,The concentration of IFN-γ, IL-9, IL-17A, IL-22 was observed to elevate. Moreover, both CXCL10+,CCL20+ cells and CCL27+cells, were adjacent to CXCR3+ cells,CCR6+cells ,CCR10+ cells respectively in Paraffin section of kidney.

Conclusion

The activity of microscopic polyangiitis could lead to abnormalitis of absolute count and subsets in CD4+T cells. CD4+ T cell is obviously activated and recruited to kidney to induce immunopathologic injury.