Abstract: SA-PO745
Probiotic Bifidobacterium animalis subsp. lactis Bi-07 Protects Intestinal Barrier by Alleviating Intestinal Oxidative Stress in Uremic Rats
Session Information
- CKD: Mechanisms - III
November 09, 2019 | Location: Exhibit Hall, Walter E. Washington Convention Center
Abstract Time: 10:00 AM - 12:00 PM
Category: CKD (Non-Dialysis)
- 2103 CKD (Non-Dialysis): Mechanisms
Authors
- Wei, Meng, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China
- Shi, Kehui, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China
- Liu, Hua, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China
- Jiang, Hongli, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China
Background
To investigate whether oral administration of probiotic Bifidobacterium animalis subsp. lactis Bi-07（B. animalis subsp. lactis Bi-07）could protect intestinal barrier in uremic rats and to explore whether it could alleviate oxidative stress in intestinal tissues.
Methods
Thirty SD rats were randomly divided into 3 groups (n=10). The sham operation group only opened the renal capsules. The uremia group underwent 5/6 nephrectomy. Uremia+probiotics group: B.animalis subsp. lactis Bi-07 was administered daily to the rats for 5 weeks after 5/6 nephrectomy. Determination of 99mTc-DTPA in blood and urine after intragastric administration of 99mTc-DTPA solution evaluated Intestinal permeability . At the end of the intervention, the intestinal segments were retrieved. The ultrastructure of the intestinal epithelial tight junction (TJ) complex was observed by scanning electron microscopy (SEM) combined with lanthanum nitrate staining. After preparation of intestinal homogenate, malondialdehyde (MDA) content and superoxide dismutase (SOD) activity were detected.
Results
SEM showed that microvilli structure of the intestinal epithelial cells in the uremia group was disordered, sparse and shed. Lanthanum nitrate dye could penetrate into the intestinal epithelial cells gap. Compared with the uremia group, microvilli were neatly arranged and TJ was structurally intact.Little of lanthanum nitrate penetrated cell gap in uremia+ probiotics group. Compared with uremic group, B. animalis subsp. lactis Bi-07 can significantly reduce intestinal permeability (P<0.05).The content of MDA of the ileal tissue was significantly increased in the uremia group (P<0.05), SOD activity was significantly decreased (P<0.05). Compared with the uremia group, the uremia +probiotic group significantly increased SOD levels, suggesting that B. animalis subsp. lactis Bi-07 protects the intestinal barrier by alleviating oxidative stress .
Conclusion
The probiotic B. animalis subsp. lactis Bi-07 can protect the intestinal barrier in uremic rats. The mechanism may be to alleviate intestinal oxidative stress and reduce intestinal barrier damage in uremia.
Funding
- Government Support - Non-U.S.