ASN's Mission

To create a world without kidney diseases, the ASN Alliance for Kidney Health elevates care by educating and informing, driving breakthroughs and innovation, and advocating for policies that create transformative changes in kidney medicine throughout the world.

learn more

Contact ASN

1401 H St, NW, Ste 900, Washington, DC 20005

email@asn-online.org

202-640-4660

The Latest on X

Kidney Week

ASN / Education & Meetings / Kidney Week /

Please note that you are viewing an archived section from 2019 and some content may be unavailable. To unlock all content for 2019, please visit the archives.

Abstract: SA-PO250

The Alteration of Non-Transferrin-Bound Iron (NTBI) and Malondialdehyde (MDA)-LDL After Single-Dose Oral Iron Administration (OIA) in Hemodialysis (HD) Patients

Session Information

Category: Anemia and Iron Metabolism

  • 202 Anemia and Iron Metabolism: Clinical

Authors

  • Saito, Noriko, Shinraku-en Hospital, Niigata, Japan
  • Saito, Kazuhide, Niigata University, Niigata, Japan
  • Shimada, Hisaki, Shinraku-en Hospital, Niigata, Japan
  • Morioka, Tetsuo, Shinraku-en Hospital, Niigata, Japan
Background

OIA is not considered to increase NTBI because of slow iron adsorption rate. Aim of this study is to assess the alteration of NTBI and an oxidative stress marker, MDA-LDL after single dose OIA.

Methods

25 HD patients without any iron load within 4 weeks, whose Hb<12g/dl, ferritin<100ng/ml and CRP<1.0mg/dl and 13 healthy volunteers received oral ferrous sulfate 105mg. 21 HD patients without OIA were as HD control.
We evaluated the following markers before and at 1, 2, 3, 4 and 48 hours(hrs) after OIA : MDA-LDL, NTBI, Hepcidin-25(HPC), serum iron(Fe), TSAT, ferritin, selenium(Se) and standard hematological parameters. Vitamin C(VC) were measured before and at 4 and 48hrs. MDA-LDL was measured by ELISA. NTBI was measured by recently described reliable method(Clin Chim Acta437:129-135, 2014).

Results

Fe, TSAT and NTBI increased after OIA and reached the peak at 4hrs, ferritin also increased at 48hrs in both HD patients and healthy control. In HD control without OIA, they did not change. NTBI and HPC basal levels were higher in healthy control than in HD patients. MDA-LDL before OIA was not different between the two groups. MDA-LDL increased from 1 to 4hrs during HD and returned to the basal level at 48 hrs irrespective of OIA, however, in healthy control, no significant alteration was observed.
In HD patients, Se level before OIA was a negative predictor for Log(MDA-LDL) before OIA by stepwise analysis(β=-0.459, p=0.021, R2=0.21).
In healthy control, NTBI before OIA was a predictor for Log(MDA-LDL) before OIA(β=0.768, p=0.002, R2=0.589).
Percentage of hypo-hemoglobinised (HypoHe) in HD patients (β=-0.443, p=0.027, R2=0.196) and HPC in healthy control (β=-0.637, p=0.019, R2=0.406) before OIA were negative predictors for the maximum level of NTBI after OIA, respectively.
Antioxydants, Se and VC basal levels were lower in HD patients. Se level did not change during HD. VC level decreased after HD and recovered at 48 hrs.

Conclusion

NTBI significantly increased after OIA. However, MDA-LDL significantly increased during HD session irrespective of OIA, whereas it did not change in healthy control after OIA. Although OIA increase NTBI, it had little influence on the MDA-LDL level. This may result of the exquisite balance of the oxydative stress and the antioxydant activity.