Abstract: SA-PO582
Remodeling of the Glomerular Tuft in Proteinuric Kidney Disease
Session Information
- Glomerular Diseases: Fibrosis, Extracellular Matrix
November 09, 2019 | Location: Exhibit Hall, Walter E. Washington Convention Center
Abstract Time: 10:00 AM - 12:00 PM
Category: Glomerular Diseases
- 1201 Glomerular Diseases: Fibrosis and Extracellular Matrix
Authors
- Liu, Michelle, Angion, Uniondale, New York, United States
- Goldberg, Itzhak D., Angion Biomedica Corp., Uniondale, New York, United States
- Narayan, Prakash, Angion Biomedica Corp, Uniondale, New York, United States
Background
Glomerular hypertrophy is associated with proteinuric kidney disease and progression to renal failure. The present study sought to quantify the association between remodeling of the glomerular tuft and proteinuria.
Methods
Adult male Wistar rats (sensitive strain, ~75 g) were administered puromycin aminonucleoside (PAN, 167 mg/kg, n=8) or water (n=3, sham cohort). On Day 14 after PAN administration, 24-hour urine was collected for determination of proteinuria. Left kidneys were removed, sectioned and stained with hematoxylin-eosin and glomeruli (14 per kidney) photographed at 40X. Areas of the Bowman’s capsule and glomeruli were measured using ImageJ by an observer blinded to the experiment groups and the area of the Bowman’s space calculated.
Results
Compared to the sham cohort, PAN-administered animals exhibited a 3-fold increase in proteinuria (p<0.01) and changes in glomerular tuft morphology (A). Significant increases (B) were observed in the areas of the Bowman’s capsule (**, p<0.01), the glomerulus (**, p<0.01), and the Bowman’s space)*, p<0.05) with PAN administration.
Conclusion
Remodeling of the glomerular tuft, including hypertrophy of the Bowman’s capsule and glomerulus, and an increase in Bowman’s space, accompanies proteinuric kidney disease. These findings might be an important consideration in the development of therapies for the treatment of kidney disease.
The PAN model of proteinuric kidney disease is associated with an increase in the area of the Bowman's capsule, hypertrophy of the glomerulus, and an increase in the Bowman's space.
Funding
- Private Foundation Support