Abstract: SA-PO427
RNA Sequencing Profile of Circular RNAs in Mouse Kidney During Aging
Session Information
- Genetic Diseases of the Kidney - III
November 09, 2019 | Location: Exhibit Hall, Walter E. Washington Convention Center
Abstract Time: 10:00 AM - 12:00 PM
Category: CKD (Non-Dialysis)
- 2103 CKD (Non-Dialysis): Mechanisms
Authors
- Gao, Fanfan, First Affiliated Hospital of Medicine School, Xi'an Jiaotong University, Xi'an, shaanxi, China
- Jiang, Hongli, First Affiliated Hospital of Medicine School, Xi'an Jiaotong University, Xi'an, shaanxi, China
Background
Kidney aging is an important clinical problem, not only because normal aging reduces renal function but also because of the high frequency of ESRD, renal cancer, and renal failure in elderly people. At the present time, the molecular basis of renal aging is not clearly known. For example, the abundance and function of circular RNAs (circRNAs) in other disease have been reported, but their alterations in the biology of renal aging remain elusive.
Methods
Renal Specimens were collected from 3-month-old and 24-month-old C57BL/6 mice. Total RNA was extracted using Trizol reagent following the manufacturer's procedure. The circRNA expression was performed using secondary Sequencing. Quantitative real-time reverse-transcription polymerase chain reaction (qRT-PCR) was used to quantify the expression of circRNAs.
Results
A total of 134 distinct circRNA candidates were detected. Among them, we defined the statistical criteria for selecting aberrant-expressed circRNA using a p-value of < 0.05 with a fold change of > 2.0 or < 0.5. A total of 86 circRNA were upregulated and 48 circRNA were downregulated significantly in the 24-month-old tissues. Furthermore, an association of the circRNA-miRNA-mRNA was investigated, showing that 17 dysregulated circRNA successfully predicted an interaction with several age-related miRNAs-mRNAs. Finally, validation of down-regulation of circRNA6456 in 24-month-old compared to 3-month-old by qRT-PCR, indicating that circRNA6456 may delay renal senescence in mice.
Conclusion
This observational study demonstrated dysregulation of circRNA in age-related kidneys, which may have an impact on development of potential biomarkers in aging.
Funding
- Government Support - Non-U.S.