Abstract: SA-PO809
Lipidomics of Feces in Hemodialysis Patients and Some Potential Biomarkers
Session Information
- Health Maintenance, Nutrition, Metabolism - II
November 09, 2019 | Location: Exhibit Hall, Walter E. Washington Convention Center
Abstract Time: 10:00 AM - 12:00 PM
Category: Health Maintenance, Nutrition, and Metabolism
- 1300 Health Maintenance, Nutrition, and Metabolism
Authors
- Qu, Ning, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China
- Jiang, Hongli, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China
- Chen, Lei, Xi'an Jiaotong University, Xi'an, China
Background
Earlier studies have demonstrated that chronic kidney disease (CKD) results in profound changes in lipid. Serum lipid profile of CKD patients has been determinated at many studies, but few studies have measured fecal lipid profile of CKD patients. To gain an in-depth insight into the fecal lipid profile of CKD, we performed a fecal lipidomics study of patients receiving hemodialysis (HD).
Methods
Ultra-performance liquid chromatography/mass spectrometry was used to investigate the fecal lipid profiles of 16 healthy controls and 26 patients receiving regular hemodialysis. Principal component analysis (PCA) and orthogonal-partial least squares analysis (OPLS-DA) were used for multivariate statistical analysis to screen potential biomarkers related to diseases.
Results
A total of 478 lipid species were identified from positive and negative ion modes. Out of 478, 52 identified lipid species were significantly altered in patients with ESRD(all P＜0.05). According to the VIP value of OPLS-DA model,46 lipid molecules with VIP > 1 were screened. There were only 4 lipid molecules satisfying the condition of VIP > 1 and P < 0.05, including DG(18:0/18:3)+NH4,TG(18:1/18:1/22:0)+NH4,TG(20:1/18:1/18:1)+NH4 and TG(18:1/18:2/22:0)+NH4.They were all decreased compared with the healthy controls. TG(18:1/18:1/22:0)+NH4,TG(20:1/18:1/18:1)+NH4 and TG(18:1/18:2/22:0)+NH4 are all triglycerides which participate in pathways including fat digestion and absorption, vitamin digestion and absorption and regulation of lipolysis in adipocytes that could be searched at the KEGG website. DG (18:0/18:3) +NH4 is diglyceride involved in adipocytokine signaling pathway which is correlated with leptin production.
Conclusion
Fecal lipidomics uncovered the lipid metabolism disorder of HD patients, especially disturbances in the gut. The 4 lipid species could be potential biomarkers of ESRD. Aa reported, the incidence rate of cardiovascular and cerebrovascular diseases (CCDs) is now increasingly high in CKD patients and high cholesterol is closely related to CKD. Our study indicated that ESRD patients had lower triglycerides and diglycerides in feces than healthy controls and we considered that CKD patients may absorb more glycerides which promote to the development of CCDs. However, the exact mechanism still needs to be studied further.