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Kidney Week

Abstract: FR-PO758

Differentiated Expression of Biomarkers for Interstitial Cells During Kidney Development

Session Information

Category: Development, Stem Cells, and Regenerative Medicine

  • 500 Development, Stem Cells, and Regenerative Medicine

Authors

  • Xing, Jia, Basic Medical College, China Medical University, Shenyang, China
  • Zhang, Jie, Basic Medical College, China Medical University, Shenyang, China
  • Wang, Kaiyue, Basic Medical College, China Medical University, Shenyang, China
  • Deng, Si-Qi, Basic Medical College, China Medical University, Shenyang, China
  • Song, Kexin, Basic Medical College, China Medical University, Shenyang, China
  • Gu, Ling, Basic Medical College, China Medical University, Shenyang, China
  • Zhai, Xiao-Yue, Basic Medical College, China Medical University, Shenyang, China

Group or Team Name

  • Nephrology laboratory
Background

Recently, the development of renal fibrosis is thought to involve 3 more cell types in addition to renal epithelial and endothelial cells. They are pericytes, mesangial cells, and fibroblasts, which are all derived from mesenchymal cells and possess the potential to differentiate into ECM-secreting cells. According to literature, the biomarkers for them are PDGFR-β, α-SMA, and desmin, however they can not be specifically identified with alone immunostaining. Therefore, a systematic study of the relationship among three biomarkers for the differentiation of the mesenchymal cells is helpful for the cytological study of renal fibrosis.

Methods

In this study, we utilized serial kidney sections with multiple developmental timepoints (E12.5, E15.5, E17.5, P1, P3, P5, P7, P14, P21, P28, and P40) and localized PDGFR-β, α-SMA, and desmin combined with CD34 by immunostaining.

Results

1. Druing the early development, PDGFR-β and α-SMA were expressed in reticular mesenchymal cells, which were perpendicular to scanty bundles of tubules and ureteric buds, while desmin was mainly expressed in cap-mesenchyme in early stage.
2. As more and more tubules extended into medulla, their expressions were decreased and confined to interstitial cells in the mature kidney, while peritubular vessels expressed CD34 instead. PDGFR-β was expressed at both fibroblast and pericyte, while α-SMA and desmin were respectively expressed at fibroblast and pericyte.
3. Through the kidney development, both PDGFR-β and α-SMA were expressed in the structure, closely associated with the artery and arteriole, with former in the outer layer (adventitia) while latter in smooth muscle cells of inner layer, next to CD34 positive endothelial cells. No co-localization was observed.
4. All of them were expressed in young glomeruli, while α-SMA vanished in the mature glomeruli.

Conclusion

Although the expressions of PDGFR-β and α-SMA are associated with renal vasculogenesis and angiogenesis, their expressions are related with different vascular structures, respectively participating in differentiation of endothelial and subendothelial smooth muscle cell, while desmin is related to the differentiation of renal progenitor cells. Among the three, PDGFR-β is the most reliable biological marker of grown renal interstitial cells, including pericytes.

Funding

  • Government Support - Non-U.S.