Abstract: TH-PO640
RNA Sequencing Analysis of Experimental Uremia and Compensated Pancreatic Exocrine
Session Information
- Health Maintenance, Nutrition, Metabolism - I
November 07, 2019 | Location: Exhibit Hall, Walter E. Washington Convention Center
Abstract Time: 10:00 AM - 12:00 PM
Category: Health Maintenance, Nutrition, and Metabolism
- 1300 Health Maintenance, Nutrition, and Metabolism
Authors
- Qu, Ning, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China
- Jiang, Hongli, First Affiliated Hospital of Medicine School, Xi'an Jiaotong University, Xi'an, China
- Chen, Lei, Xi'an Jiaotong University, Xi'an, China
Background
Malnutrition is a common problem in patients with chronic kidney disease (CKD). About 30 years ago, several studies showed that more than half of patients with end-stage renal disease (ESRD) suffered from exocrine pancreatic insufficiency. Recent years, some studies also reported this phenomenon. But it’s not clear that if there is compensatory mechanism to help relieve the symptom.
Methods
Sprague-Dawley rats were divided into two groups: sham (group NC) and uremia (group U). Uremia group rats were processed with 5/6 nephrectomy for chronic renal failure. All rats were fed for 24 weeks. High-throughput sequencing technology was used to detect all gene expression differences in jejunum tissue samples of all rats. Differentially expressed genes were screened out and through functional annotation and enrichment analysis, gene expression signaling pathways of sham group rats and uremia group rats were screened out.
Results
The results showed that 95 up-regulated genes and 18 down-regulated genes were screened in uremia group compared with sham group. Analysis of GO function and KEGG signaling pathway enrichment showed that the above differentially expressed genes were mainly enriched in protein digestion and absorption, pancreatic secretion, fat digestion and absorption and other functions and pathways. The pancreatic secretion signaling pathway was related to our aim. RNA expressions of many genes increased including prss1,prss2,prss3,cpa1,cpa2,cpb1,cela3b,cela2a,ctrl,ctrb1,pla2g1b,pnlip,pnliprp1 and cel and these genes all code digestive enzymes like trypsin, carboxypeptidase, pancreatic elastase, chymotrypsin and phospholipase. Except trypsin, the enzymes above are usually detected by pancreatic secretion while their RNA expressions increased in the jejunum.
Conclusion
Patients with ESRD suffered from exocrine pancreatic insufficiency but we found that some pancreatic secreted enzymes increased RNA expressions in the jejunum of experimental uremia rats. So we can deduce that the jejunum could more or less compensate for exocrine pancreatic insufficiency of ESRD patients. However, the exact mechanism still needs to be studied further.