ASN's Mission

ASN leads the fight to prevent, treat, and cure kidney diseases throughout the world by educating health professionals and scientists, advancing research and innovation, communicating new knowledge, and advocating for the highest quality care for patients.

learn more

Contact ASN

1401 H St, NW, Ste 900, Washington, DC 20005

email@asn-online.org

202-640-4660

The Latest on Twitter

Kidney Week

Abstract: SA-PO061

Circular RNA Expression Profiles in Cisplatin-Induced AKI in Mice

Session Information

Category: Acute Kidney Injury

  • 103 AKI: Mechanisms

Authors

  • Li, Canming, The Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou, China
  • Li, Ming, The Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou, China
  • Ye, Zengchun, The Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou, China
  • Peng, Hui, The Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou, China
  • Lou, Tan-qi, The Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou, China
Background

Cisplatin is an effective chemotherapeutic agent whose nephrotoxicity is a serious clinical problem. However, the molecular mechanisms underlying cisplatin-induced acute kidney injury (Cis-AKI) remain unknown. Circular RNAs (circRNAs), a novel class of noncoding RNAs, have been reported to be involved in a variety of diseases. However, the roles of circRNAs in AKI are poorly understood.

Methods

In this study, an AKI model was established in cisplatin-treated mice, and the expression of circRNAs was profiled by next-generation sequencing. The differential expression levels of selected circRNAs were determined by qRT-PCR. Bioinformatics analysis was conducted to predict the functions.

Results

In total, 368 circRNAs were detected to be differentially expressed in response to cisplatin treatment. The qRT-PCR analysis showed that the expression of six selected circRNAs was consistent with that determined by RNA sequencing. The GO and KEGG pathway analyses indicated that the parental genes of the differentially expressed circRNAs were predominantly implicated in the cell part and organelle, cellular process, metabolic process and cancer pathways.

Conclusion

Our study yielded a comprehensive expression profile of differentially expressed circRNAs associated with AKI, indicating the possible involvement of these dysregulated circRNAs in the pathophysiology of cisplatin-induced nephrotoxicity.

Funding

  • Government Support - Non-U.S.