Abstract: SA-PO061
Circular RNA Expression Profiles in Cisplatin-Induced AKI in Mice
Session Information
- AKI: Mechanisms - Primary Injury and Repair - II
November 09, 2019 | Location: Exhibit Hall, Walter E. Washington Convention Center
Abstract Time: 10:00 AM - 12:00 PM
Category: Acute Kidney Injury
- 103 AKI: Mechanisms
Authors
- Li, Canming, The Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou, China
- Li, Ming, The Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou, China
- Ye, Zengchun, The Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou, China
- Peng, Hui, The Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou, China
- Lou, Tan-qi, The Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou, China
Background
Cisplatin is an effective chemotherapeutic agent whose nephrotoxicity is a serious clinical problem. However, the molecular mechanisms underlying cisplatin-induced acute kidney injury (Cis-AKI) remain unknown. Circular RNAs (circRNAs), a novel class of noncoding RNAs, have been reported to be involved in a variety of diseases. However, the roles of circRNAs in AKI are poorly understood.
Methods
In this study, an AKI model was established in cisplatin-treated mice, and the expression of circRNAs was profiled by next-generation sequencing. The differential expression levels of selected circRNAs were determined by qRT-PCR. Bioinformatics analysis was conducted to predict the functions.
Results
In total, 368 circRNAs were detected to be differentially expressed in response to cisplatin treatment. The qRT-PCR analysis showed that the expression of six selected circRNAs was consistent with that determined by RNA sequencing. The GO and KEGG pathway analyses indicated that the parental genes of the differentially expressed circRNAs were predominantly implicated in the cell part and organelle, cellular process, metabolic process and cancer pathways.
Conclusion
Our study yielded a comprehensive expression profile of differentially expressed circRNAs associated with AKI, indicating the possible involvement of these dysregulated circRNAs in the pathophysiology of cisplatin-induced nephrotoxicity.
Funding
- Government Support - Non-U.S.