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Abstract: SA-PO299

Water Deprivation Shortens Primary Cilia Length in the Kidney Tubular Cells

Session Information

Category: Fluid and Electrolytes

  • 901 Fluid and Electrolytes: Basic

Authors

  • Kong, Min Jung, Kyungpook National University, Daegu, Korea (the Republic of)
  • Kim, Jee in, Keimyung University, Daegu, Korea (the Republic of)
  • Park, Kwon Moo, Kyungpook National University, Daegu, Korea (the Republic of)
Background

The primary cilium, a microtubule-based cellular organelle, plays a key regulator for maintenance of cell homeostasis by sensing and transducing extracellular signals. In the kidney, the length of primary cilium links to the number of human kidney diseases. Here, we investigated whether water deprivation affects the primary cilium homeostasis and its underlying mechanisms in the kidney tubule cells.

Methods

C57BL6 mice were dehydrated for 1 or 2 days. Some mice were administrated with tubastatin A (an inhibitor of histone deacetylase 6, HDAC6) or saline before restriction of drinking water supply.

Results

In this study, water deprivation significantly shortened primary cilia length in kidney tubular cells in mice along with increasing urine osmolality. The kidneys derived from water-restricted mice presented low levels of acetylated-α-tubulin, EXOC5, an exocyst complex, and α-tubulin transferase expression. In Madin-Darby canine kidney (MDCK) cells, high concentrations of NaCl or mannitol treatments shortened primary cilia length. This NaCl or mannitol treatment decreased the expression of acetylated-α-tubulin, EXOC5, and α-tubulin transferase. Treatment of tubastatin A prevented drinking water deprivation-induced shortening of primary cilia in the mice. In addition, this HDAC6 inhibitor treatment prevented the decrease of acetylated-α-tubulin, EXOC5, and α-tubulin transferase expression.

Conclusion

These findings demonstrate that the length of primary cilium in kidney tubule cells is associated with water supply and urine osmolality, suggesting that primary cilium may play an important role in body water homeostasis and regulation of urine osmolality.