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Abstract: SA-PO403

Investigation of Urinary Exosomal miRNA and lncRNA Expression Profiles and ceRNA Network in Diabetic Kidney Disease

Session Information

Category: Diabetic Kidney Disease

  • 701 Diabetic Kidney Disease: Basic

Author

  • Tang, Wenbin, Xiangya Hospital Central South University Department of Nephrology, Changsha, Hunan, China
Background

Diabetic kidney disease (DKD) is one of the major cause of end-stage renal disease (ESRD), it is important to detect diagnostic biomarkers of DKD. This study aims to explore expression profiles of urinary exosomal miRNA and lncRNA in DKD patients, and their possible regulatory mechanism in DKD.

Methods

We performed urinary exosomal miRNA and lncRNA profile in DKD, DM patients and healthy subjects, and analyzed biological functions of differentially expressed miRNAs and lncRNAs. And then we focused on the important mRNA ZEB1/2 which were related to DKD, constructed a ceRNA network.

Results

We identified differentially expressed 47 miRNAs and 61 lncRNAs of urinary exosome in DKD. GO and KEGG analysis showed target genes of miRNAs were involved in Iκ-BK/NF-κB, Wnt, PI3K-Akt pathway, autophagy and oxidation-reduction process. Differential lncRNAs participated in occurrence and development of DKD through peroxisome proliferator-activated receptor, cGMP-PKG pathway, autophagy and platelet activation. Furthermore, We constructed a ceRNA network with differentially expressed miRNAs, lncRNAs, ZEB1, ZEB2, and found that 7 lncRNA-miRNA- mRNA axes could promote the expression of ZEB1/ZEB2, which may play an important role in TIF of DKD.

Conclusion

In conclusion, Urinary exosomal lncRNA-miRNA may involved in the process of TIF in DKD through targeting transcription factor ZEB1/ZEB2, providing new directions to further clarify the pathogenesis and therapeutic targets of DKD.

Funding

  • Government Support – Non-U.S.