Significance of Non-B Cell-Derived Immunoglobulin G in the Renal Tubulointerstitium of Chinese Patients with Type 2 Diabetic Kidney Disease
- Diabetic Kidney Disease: Clinical - I
November 02, 2023 | Location: Exhibit Hall, Pennsylvania Convention Center
Abstract Time: 10:00 AM - 12:00 PM
Category: Diabetic Kidney Disease
- 702 Diabetic Kidney Disease: Clinical
- Wang, Xinyao, Peking University Third Hospital, Beijing, China
- Deng, Zhenling, Peking University Third Hospital, Beijing, China
- Wang, Yue, Peking University Third Hospital, Beijing, China
Epithelial-mesenchymal transition (EMT) is critical in the progression of DKD. Studies have reported that positive IgG staining in renal tubules in patients with DKD, but the significance is unknown. Studies have demonstrated that renal intrinsic cells, including renal tubular epithelial cells, can produce IgG (non-B IgG). Therefore, we clarified the non-B IgG in the tubulointerstitium of patients with DKD and its clinicopathological features, and may contribute to renal tubule EMT.
A total of sixty patients with type 2 diabetes mellitus and biopsy-proven DKD was studied retrospectively.The monoclonal antibody RP215 can screen out human non-B IgG rather than B-IgG. The clinicopathological features of non-B IgG deposition in the renal tubulointerstitium were compared with and without. The risk factors for poor renal function and interstitial lesions were evaluated using logistic regression analysis. In addition, the relationship between tubulointerstitial non-B IgG deposition and EMT was assessed in 30 patients with DKD.
The non-B IgG positive patients showed decreased eGFR (p=0.038) and more severe anemia(p=0.002). Multivariate logistic regression analysis showed that tubulointerstitial non-B IgG deposition was an independent risk factor for poor renal function(p=0.037) and interstitial lesions(p=0.030). The positive area of non-B IgG in DKD renal tubulointerstitium and α-SMA showed a positive correlation (p=0.034).
Renal tubulointerstitial non-B IgG deposition in patients with DKD showed poorer renal function and severe interstitial lesions. The renal tubule EMT was more sever in positive tubulointerstitial non-B IgG staining, suggesting that non-B IgG may be involved in DKD kidney injury by promoting EMT.
- Government Support – Non-U.S.