ASN's Mission

To create a world without kidney diseases, the ASN Alliance for Kidney Health elevates care by educating and informing, driving breakthroughs and innovation, and advocating for policies that create transformative changes in kidney medicine throughout the world.

learn more

Contact ASN

1401 H St, NW, Ste 900, Washington, DC 20005

email@asn-online.org

202-640-4660

The Latest on X

Kidney Week

Abstract: SA-PO966

Identification of Biomarkers for Minimal Change Disease Based on LMD-DIA Technology Quantitative Proteomics

Session Information

Category: Glomerular Diseases

  • 1402 Glomerular Diseases: Clinical, Outcomes, and Trials

Authors

  • Liu, Fanna, nephrology, Guangzhou, Guangdong Province (GUP), China
  • Chen, Sining, Nephrology, Guangzhou, Guangdong Province (GUP), China
Background

Minimal change disease (MCD) accounts for 80%-90% of idiopathic nephrotic syndrome in children and 10%-15% in adults. There is no apparent pathological change in the kidney tissue under the light microscope, but the podocyte foot process is diffusely disappeared under the electron microscope.

Methods

The kidney tissues of 5 MCD patients and 5 normal patients were selected as the experimental and control group. The three regions of kidney: the glomerulus, renal tubule, and renal interstitium were sequentially cut by using a laser microdissection device. The fold change ≥1.5 was used as the criterion of significant difference to screen different proteins. GO enrichment analysis, KEGG pathway analysis, Gene Set Enrichment Analysis and PPI analysis were performed on the identified differential proteins to determine the target protein.

Results

We found that 552 differentially expressed proteins (DEPs) in the glomerulus group, 61 DEPs in renal interstitium group, 108 DEPs in renal tubular group. After bioinformatics analysis of the DEPs in the glomerular group, we found that the oxidative phosphorylation pathway and regulation of actin cytoskeleton pathway were play a significant role in MCD. In the renal interstitium group, Heterogeneous nuclear ribonucleoprotein K (hnRNP-K) were the most up-regulated DEPs and 4-hydroxyphenylpyruvate dioxygenase (HPD) were most down-regulated DEPs. In renal tubules group. Down-regulated HSP90AB1 have great influence in Akt to increase the apoptosis of renal tubule cell by PI3K−Akt signaling pathway.

Conclusion

ACTN4, ZYX, CSK and CAN1 are potential biomarkers for early diagnosis of MCD. COPD, HPRT, FIBG, HEMO, GTR1 and WBP2 may be the key proteins interacting with the immune compartment of the MCD kidney.

Funding

  • Government Support – Non-U.S.