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Kidney Week

Abstract: SA-PO943

Urinary Exosomal miRNA Signature of IgA Nephropathy: A Case-Control Study

Session Information

Category: Glomerular Diseases

  • 1402 Glomerular Diseases: Clinical, Outcomes, and Trials

Author

  • Shankar, Mythri, Institute of Nephro Urology, Bangalore, Karnataka, India
Background

IgAN is a progressive disease. The current gold standard for diagnoses is kidney biopsy-invasive procedure with life-threatening complications. The hour needs a non-invasive, reliable, stable & accurate biomarker.miRNAs are small, non-coding endogenous RNA.Urinary exosomes are rich in miRNA and can be used as novel non-invasive biomarkers.We aim to study the urinary exosomal miRNA signature of pts with IgAN.

Methods

50 biopsy-proven IgAN pts & 50 healthy controls were recruited in India. Urinary exosomes were isolated & miRNA extracted. Analysis was done with digital multiplexed nCounter® human miRNA Expression Assay which contains 799 unique miRNA barcodes. Lasso regression & consensus clustering were performed to discover significant miRNA.

Results

Mean age:36.8yrs. Male:Female=3:1. Mean creatinine & proteinuria : 2.2 mg/dl and 2.9 gms/day respectively. Majority miRNAs were significantly downregulated. A group of 5 candidate miRNAs hsa.miR.146b.3p+hsa.miR.599+hsa.miR.4532+hsa.miR.664b.5p+hsa.miR.221.5p which could successfully differentiate between IgAN cases and healthy controls with very high sensitivity and specificity.

Conclusion

The group of five candidate urinary exosomal miRNAshsa.miR.146b.3p+hsa.miR.599+hsa.miR.4532+hsa.miR.664b.5p+hsa.miR.221.5p has the potential to serve as novel non-invasive biomarkers for IgA nephropathy.

Model using the group of 5 miRNAs for diagnosis
Accuracy (95% CI)1 (0.7151, 1)
Sensitivity1
Specificity1
Positive Predictive Value1
Negative Predictive Value1
Prevalence0.18
Detection Rate0.18
Detection Prevalence0.18
AUC1
Number of misclassified samples0
Wrongly classified samplesNA
Kappa1

Heat map showing the 5 miRNAs with biomarker potential giving a accuracy of 99%

Funding

  • Government Support – Non-U.S.