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Kidney Week

Abstract: SA-PO156

Reno-Protective Effect of Ginsenoside Rg3 in Ischemia Reperfusion Injury Mice by Inducing Autophagy Flux

Session Information

  • AKI: Mechanisms - III
    November 04, 2023 | Location: Exhibit Hall, Pennsylvania Convention Center
    Abstract Time: 10:00 AM - 12:00 PM

Category: Acute Kidney Injury

  • 103 AKI: Mechanisms

Authors

  • Han, Suyeon, Chungnam National University Hospital, Daejeon, Korea (the Republic of)
  • Park, Kyungho, Chungnam National University Hospital, Daejeon, Korea (the Republic of)
  • Park, Heewon, Chungnam National University Hospital, Daejeon, Korea (the Republic of)
  • Hwang, Yunkyeong, Chungnam National University Hospital, Daejeon, Korea (the Republic of)
  • Park, Hye Rim, Department of Medical Science, Chungnam National University, Daejeon, Korea (the Republic of)
  • Lee, Eu Jin, Chungnam National University Hospital, Daejeon, Korea (the Republic of)
  • Kim, Hae Ri, Chungnam National University Sejong Hospital, Sejong, Korea (the Republic of)
  • Ham, Youngrok, Chungnam National University Hospital, Daejeon, Korea (the Republic of)
  • Na, Kiryang, Chungnam National University Hospital, Daejeon, Korea (the Republic of)
  • Lee, Kang Wook, Chungnam National University Hospital, Daejeon, Korea (the Republic of)
  • Choi, Dae Eun, Chungnam National University Hospital, Daejeon, Korea (the Republic of)
Background

The protective effects of Ginsenoside Rg3 (Rg3) have been demonstrated through different mechanisms. Nevertheless, the specific evaluation of its renal-protective effect and the involvement of autophagy remain unclear. This study aims to examine how Rg3 induces autophagy flux and diminishes renal cell death in renal ischemia reperfusion injury (IRI).

Methods

C57Bl/6 mice were categorized into the subsequent groups: sham; sham treated with Rg3; IRI mice treated with saline; IRI mice treated with Rg3. Kidneys and blood samples were obtained 24 hours after the surgical procedure (sham and IR operation). Renal function, kidney histology, and the protein expression of autophagy markers were assessed.

Results

In the IRI mice group, there was an elevation of BUN and s-Cr levels compared to the sham group. However, the administration of Rg3 resulted in a reduction of BUN and s-Cr levels in the IRI mice. Furthermore, Rg3 treatment led to a decrease in renal injury, as evidenced by a lower renal tubular cell detachment and necrosis score in the IRI mice. The Rg3-treated IRI mice exhibited reduced oxidative stress and improved autophagy, characterized by increased levels of LC3 and Beclin-1, decreased levels of p62, and higher levels of renal ATP6E compared to the IRI mice treated with saline. Additionally, Rg3 treatment promoted the phosphorylation of AMPK in the kidneys of IRI mice.

Conclusion

Rg3 exhibits renal protection against renal IRI by enhancing autophagy flux.