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Kidney Week

Abstract: FR-PO537

Resveratrol Inhibits Proximal Tubular Sodium Reabsorption and Suppresses the Progression of Renal Injury

Session Information

Category: Fluid, Electrolytes, and Acid-Base Disorders

  • 1101 Fluid, Electrolyte, and Acid-Base Disorders: Basic

Authors

  • Nakamura, Motonobu, Division of Nephrology and Endocrinology, The University of Tokyo, Bunkyo,, Tokyo, Japan
  • Satoh, Nobuhiko, Division of Nephrology and Endocrinology, The University of Tokyo, Bunkyo,, Tokyo, Japan
  • Takagi, Mayuko, Division of Nephrology and Endocrinology, The University of Tokyo, Bunkyo,, Tokyo, Japan
  • Nangaku, Masaomi, Division of Nephrology and Endocrinology, The University of Tokyo, Bunkyo,, Tokyo, Japan
Background

Resveratrol(RSV), a natural polyphenol compound, has demonstrated anti-inflammatory, anti-cancer, anti-oxidant, and anti-aging effects in vitro and in vivo studies. However, its effects on the proximal tubules (PTs) and kidney disease remains unknown. In this study, we evaluated the effect of RSV on Na transport in isolated PTs and its potential to inhibit the progression of chronic renal injury in rat models.

Methods

By using a pH-sensitive dye BCECF we measured the basolateral Na+-HCO3- Cotransporter (NBCe) 1 activity and apical Na+-H+ Exchanger (NHE) activity in freshly-isolated rat PTs. To evaluate the effect of RSV on the progression of kidney disease, 6-week-old OLETF rats that had undergone unilateral nephrectomy were fed a high-salt diet, administered aldosterone (Ald) continuously by osmotic pump, and compared to a group receiving 50 mg/kg BW RSV orally daily for four weeks. Comparisons were made by serum biochemical, histological tests and protein expression. Protein expression was determined by Western blot.

Results

In a concentration-dependent manner, RSV significantly inhibited the stimulatory effects of insulin on Na reabsorption on PTs, and inhibited insulin-induced phosphorylation of Akt. Moreover, continuous Ald administration resulted in severe albuminuria and renal dysfunction, whereas those treated with RSV exhibited a significant reduction in both albuminuria (p < 0.05) and renal dysfunction (p < 0.05). In the renal cortex, phosphorylation of Akt and expression of Rictor and Raptor were significantly decreased (p < 0.05).

Conclusion

RSV inhibits insulin-induced sodium reabsorption in PTs, and it could have a renoprotective effect.

Funding

  • Government Support – Non-U.S.