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Abstract: SA-PO1031

Longitudinal Analysis of Urine Metabolomics of Preterm Infants Based on Ultra-High-Performance Liquid Chromatography-Tandem Mass Spectrometry (UHPLC-MS/MS) Technique

Session Information

Category: Pathology and Lab Medicine

  • 1800 Pathology and Lab Medicine


  • Shen, Qian, Children's Hospital of Fudan University, Shanghai, China
  • Xu, Hong, Children's Hospital of Fudan University, Shanghai, China
  • Hong, Sha, Children's Hospital of Fudan University, Shanghai, China

To explore the regular urinary metabolomic characteristics of preterm infants in the early postnatal period and find the association between early metabolic adaptation and urinary metabolism.


Between June 1, 2021 and September 31, 2022, 36 preterm infants were recruited from the Children’s Hospital of Fudan University, urine samples were collected on postnatal day 1 (PND1), PND7) and PND14 respectively, for a longitudinal cohort self-control analysis. UHPLC-MS/MS was used to characterize urinary metabolomic changes at postnatal week 1 and week 2. Multivariate analyses were used to screen for differential metabolites and metabolic pathway.


Widely targeted metabolomic analysis of urine from the first week of postnatal life in preterm infants identified 57 metabolites whose levels were upregulated and 18 metabolites that were downregulated from birth(Fig1). Widely targeted metabolomic analysis of urine from preterm infants at postnatal week 2 revealed that urine levels of 22 metabolites were upregulated and 7 metabolites were downregulated compared to week 1. Metabolic pathways significantly enriched in 29 urinary differential metabolites in the second postnatal week in preterm infants, the gluconeogenesis/glycolysis pathway was the metabolic pathway significantly associated with all urinary differential metabolites in the first 2 postnatal weeks (Fig2).


Urinary metabolic changes in preterm newborns were more pronounced in the 1st week after birth than in the 2nd week, suggesting metabolic adaptation in preterm newborns occurs mainly in the 1st week after birth. Glycometabolism is the key to the changes in urine metabolism in preterm infants during the first 2 weeks of life, and it is hypothesized that the regulation of glucose homeostasis in preterm infants is the focus of their early metabolism and development.