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Kidney Week

Abstract: FR-PO175

Protective Effect of Piperazine Ferulate on AKI in Septic Rats

Session Information

  • AKI: Mechanisms - II
    November 03, 2023 | Location: Exhibit Hall, Pennsylvania Convention Center
    Abstract Time: 10:00 AM - 12:00 PM

Category: Acute Kidney Injury

  • 103 AKI: Mechanisms

Authors

  • Liu, Xinyu, Nanyang Central Hospital, Nanyang, Henan, China
  • Wang, Jiangang, Nanyang Central Hospital, Nanyang, Henan, China
  • Li, Di, Nanyang Central Hospital, Nanyang, Henan, China
  • Deng, Mengyuan, Nanyang Central Hospital, Nanyang, Henan, China
  • Li, Qianqian, Nanyang Central Hospital, Nanyang, Henan, China
  • Ding, Xingxing, Nanyang Central Hospital, Nanyang, Henan, China
  • Liu, Qi, Nanyang Central Hospital, Nanyang, Henan, China
Background

Ferulic acid piperazine is widely used in chronic nephritis and nephrotic syndrome in clinical practice. The aim of this study was to investigate the role of ferulic piperazine (PF) in sepsis-induced acute kidney injury (AKI) rats and its mechanism.

Methods

The sepsis-induced AKI model in male SD rats was prepared by cecum ligation puncture (CLP) method. The animals were randomly divided into five groups: normal group, sham-operated group, CLP group and two PF-treated groups, and PF was administered by gavage at a dose of 50 and 100 mg / kg, respectively. Renal index, serum creatinine, blood urea nitrogen and NGAL were measured to evaluate renal function. PAS staining was used to observe renal histopathological changes. Serum inflammatory mediators (PCT, IL-6) levels were measured to assess serum inflammation levels; oxidative stress indicators (T-SOD, CAT, MDA, GSH-PX) were measured to assess body redox activity and oxidative stress levels. western blot was used to detect the expression levels of nuclear factor E2-related factor 2 (Nrf2) protein and au-rich element (ARE) binding factor 1 ( AUF1) protein expression levels.

Results

PF treatment significantly reduced renal index and serum creatinine, urea nitrogen, and NGAL levels in rats.PF was also effective in ameliorating renal pathological damage. PF treatment was observed to suppress renal inflammation by reducing PCT and IL-6 levels. PF treatment restored the decrease in serum GSH-PX (glutathione peroxidase), CAT (catalase) and T-SOD (total superoxide dismutase), while also modulating the elevation of MDA (malondialdehyde). In addition, PF treatment significantly enhanced the expression of Nrf2 and AUF1 proteins in the kidney.

Conclusion

PF has a potential therapeutic effect on AKI in septic rats, and its activity may be related to the activation of anti-inflammatory and antioxidant effects of Nrf2 and AUF1 proteins.

Funding

  • Clinical Revenue Support