Abstract: TH-PO800
Intersectin 1 and Intersectin 2 Localize with IgG Deposition in Glomeruli
Session Information
- Pathology and Lab Medicine - I
November 02, 2023 | Location: Exhibit Hall, Pennsylvania Convention Center
Abstract Time: 10:00 AM - 12:00 PM
Category: Pathology and Lab Medicine
- 1800 Pathology and Lab Medicine
Authors
- Kumagai, Naonori, Fujita Ika Daigaku, Toyoake, Aichi, Japan
- Kondo, Tomomi, Fujita Ika Daigaku, Toyoake, Aichi, Japan
- Matsumoto, Yuji, Fujita Ika Daigaku, Toyoake, Aichi, Japan
- Ikezumi, Yohei, Fujita Ika Daigaku, Toyoake, Aichi, Japan
Background
Intersectin 1 (ITSN1) and intersectin 2 (ITSN2) are multifunctional proteins and are causative gene for steroid-sensitive nephrotic syndrome. However, their expression in glomeruli remains unclear. IgG deposition cause glomerular injury and play a pivotal role in glomerular diseases such as membranous nephropathy and lupus nephritis. However, the molecules associated with IgG deposition remain unclear.
Methods
ITSN, ITSN2, and IgG were co-stained with immunofluorescence staining for renal biopsy specimens of pediatric patients with glomerular diseases such as steroid sensitive nephrotic syndrome, steroid resistant nephrotic syndrome, IgA nephropathy, Henoch-Schonlein nephropathy, membranous nephropathy, and lupus nephritis.
Results
ITSN1 and ITSN2 stained at the same site and with the same intensity as IgG regardless of the type of disease. In membranous nephropathy and lupus nephritis, ITSN1 and ITSN2 stained strongly at the IgG stained site with the same intensity as IgG, especially in glomerular capillary. In other kidney diseases such as IgA nephropathy, Henoch-Schonlein nephropathy, steroid sensitive nephrotic syndrome, and steroid resistant nephrotic syndrome, ITSN1 and ITSN2 stained weakly at the IgG stained site with the same intensity as IgG.
Conclusion
ITSN1 and ITSN2 seem to express in glomeruli in response to IgG deposition, since they stain at the IgG stained site with the same intensity as IgG regardless of the type of disease. They are considered commonly associated with IgG deposition regardless of the type of disease, which cause glomerular injury. It remains to elucidate the pathophysiological role of ITSN1 and ITSN2 in glomerular injury caused by IgG deposition.
Funding
- Government Support – Non-U.S.