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Abstract: FR-PO728

Role of Urine Exosomal Proteome for Identifying Potential Biomarkers to Predict Renal Allograft Survival

Session Information

  • Transplantation: Basic
    November 03, 2023 | Location: Exhibit Hall, Pennsylvania Convention Center
    Abstract Time: 10:00 AM - 12:00 PM

Category: Transplantation

  • 2101 Transplantation: Basic

Authors

  • Bhargava, Vinant, Sir Gangaram Hospital, New Delhi, Delhi, India
  • Bhalla, Anil, Sir Gangaram Hospital, New Delhi, Delhi, India
  • Gupta, Ashwani, Sir Gangaram Hospital, New Delhi, Delhi, India
  • Malik, Manish, Sir Gangaram Hospital, New Delhi, Delhi, India
  • Gupta, Anurag, Sir Gangaram Hospital, New Delhi, Delhi, India
  • Tiwari, Vaibhav, Sir Gangaram Hospital, New Delhi, Delhi, India
  • Chadha, Shiv, Sir Gangaram Hospital, New Delhi, Delhi, India
Background


Urine contains proteins bound to extracellular vesicles (exosomes) which are secreted by the cells into the urine as a result of renal insult. We propose to identify exosomal protein markers in urine to predict allograft survival. Molecule-bearing urinary exosome is altered in various kidney diseases. Exosomal molecules such as Aquaporins (AQPs) can be used as potential biomarkers.

Methods

Post renal transplant patient urine samples at 6/7th day were collected using standard protocol. Isolation of exosomes from urine samples was done using standardized protocol. Urine samples were collected at 3 , 6, 12 months or/and at the time of graft dysfunction. Further, characterization of exosomal proteins was done through Flow cytometry, Western blotting and NTA (Nano-particle tracking analysis). Exosomal proteome was analysed through LC-MS.

Results

Samples were collected from13 renal allograft patients and 14 healthy controls. There was decreased abundance of urinary exosomal aquaporin-1 observed in allograft renal transplant recipients in the immidiate post transplant and in those with allograft dysfunction ( 6/13 patients) as compared to healthy individuals. All the 6 patients underwent biopsy and demonstrated rejection ( ABMR :2, Tcellrejection: 1), CNI toxicity (n= 2), BKVN ( n=1). 24 months followup is underway.

Conclusion

The preliminary analysis of Mass Spectrometry data shows that AQP1 levels are highly downregulated in allograft patients in the immidiate post transplant period and those with allograft dysfunction. This suggests that the excretion of Urinary exosomes UE-AQP1 is altered under the conditions of renal insult. Segregating and co-relating the differential proteome data with clinical parameters and demographic features will help us to define biomarkers specific to a particular cause of allograft dysfunction in our ongoing analysis

Preliminary analysis of mass spectrometry data