Abstract: FR-PO736
Kidney Storage at Subzero Temperature Is Safe for Porcine Auto-Transplantation: A World First In Vivo Study
Session Information
- Transplantation: Basic
November 03, 2023 | Location: Exhibit Hall, Pennsylvania Convention Center
Abstract Time: 10:00 AM - 12:00 PM
Category: Transplantation
- 2101 Transplantation: Basic
Authors
- Calderon Novoa, Francisco M., University Health Network, Toronto, Ontario, Canada
- Chu, Tunpang, University Health Network, Toronto, Ontario, Canada
- Kawamura, Masataka, University Health Network, Toronto, Ontario, Canada
- Lees, Kaitlin, Sick Kids Foundation, Toronto, Ontario, Canada
- Ganesh, Sujani, University Health Network, Toronto, Ontario, Canada
- Hobeika, Christian, University Health Network, Toronto, Ontario, Canada
- Ray, Samrat, University Health Network, Toronto, Ontario, Canada
- Parmentier, Catherine, University Health Network, Toronto, Ontario, Canada
- Nogueira, Emmanuel, University Health Network, Toronto, Ontario, Canada
- Martínez-Arenas, Laura, University Health Network, Toronto, Ontario, Canada
- Conliffe, Rachael K., University Health Network, Toronto, Ontario, Canada
- Robinson, Lisa, Sick Kids Foundation, Toronto, Ontario, Canada
- Selzner, Markus, Toronto General Hospital, Toronto, Ontario, Canada
Group or Team Name
- Toronto Organ Preservation Lab.
Background
Static cold storage (SCS) at 4°C remains the method of choice for kidney preservation prior to transplantation,but the rapid decline of graft quality at 4°C limits prolonged SCS and graft exchange over larger distances. Kidney storage below 0°C could prolong graft viability and offer new opportunities for kidney graft exchange over larger distances, or controlled scheduling of kidney transplant. The aim of this study was to determine the feasibility of sub-zero storage followed by auto-transplantation of porcine kidneys.
Methods
Kidneys were retrieved from Yorkshire pigs and either stored at 4°C for five hours (n=4) or flushed and stored using a novel preservation solution (CrS SC 0.3 EQ, Cryostasis Inc®) at –2°C (n=4). After storage, kidneys were auto-transplanted.
Results
All kidneys were successfully transplanted and immediately produced urine. Results were compared between the two groups, and assesed using T-test or ANOVA. Creatinine values at days 1, 3 and 7 for the -2°C and 4°C groups were 3.07 vs. 3.05 mg/dL, 3.28 vs. 2.84 mg/dL, and 1.4 vs.1.54 mg/dL, respectively (p>0.05). 24-hour urine output was 1700 ml (562.5 - 3250 ml) vs. 1700 ml (1450- 2650 ml) for -2°C and 4°C groups. Lactate values at 1, 3 and 7 days were 0.9 vs. 1.1, 0.75 vs. 0.56, and 0.95 vs. 0.88 mmol/L (p= 0.34). AST levels at 1, 3 and 7 days were: 152.3 vs.101.6, 33.3 vs. 27.8 and 25 vs. 22.8 U/L (p>0.05). Potassium levels were 4.8 vs. 4.2, 4.3 vs. 3.8 and 4.2 vs. 4.4 mmol/L at days 1, 3 and 7 (p=0.05).
Conclusion
Sub-zero short storage of porcine kidneys is feasible and results are comparable to ideal heartbeating-donor kidneys briefly stored at 4 °C. Histological, celular and molecular analyses are underway to better understand sub-zero protective mechanisms, and its extents will be further studied by prolonging storage times.
Funding
- Commercial Support – Cryostasis Incorporated