Abstract: FR-PO382
Significance of the Nav 1.8 Voltage-Gated, Tetrodotoxin-Resistant Sodium Channel for Renal Sensoric Innervation in Mice and Rats
Session Information
- Hypertension and CVD: Basic
November 03, 2023 | Location: Exhibit Hall, Pennsylvania Convention Center
Abstract Time: 10:00 AM - 12:00 PM
Category: Hypertension and CVD
- 1601 Hypertension and CVD: Basic
Authors
- Rodionova, Kristina, Friedrich-Alexander-Universitat Erlangen-Nurnberg, Erlangen, Bayern, Germany
- Ditting, Tilmann, Friedrich-Alexander-Universitat Erlangen-Nurnberg, Erlangen, Bayern, Germany
- Holzmann, Kathrin, Friedrich-Alexander-Universitat Erlangen-Nurnberg, Erlangen, Bayern, Germany
- Bach, Felix Ralph Wolfgang, Friedrich-Alexander-Universitat Erlangen-Nurnberg, Erlangen, Bayern, Germany
- Cordasic, Nada, Friedrich-Alexander-Universitat Erlangen-Nurnberg, Erlangen, Bayern, Germany
- Hilgers, Karl F., Friedrich-Alexander-Universitat Erlangen-Nurnberg, Erlangen, Bayern, Germany
- Linz, Peter, Friedrich-Alexander-Universitat Erlangen-Nurnberg, Erlangen, Bayern, Germany
- Schiffer, Mario, Friedrich-Alexander-Universitat Erlangen-Nurnberg, Erlangen, Bayern, Germany
- Amann, Kerstin U., Friedrich-Alexander-Universitat Erlangen-Nurnberg, Erlangen, Bayern, Germany
- Veelken, Roland, Friedrich-Alexander-Universitat Erlangen-Nurnberg, Erlangen, Bayern, Germany
Background
Our previous work repeatedly showed that the sensory innervation of the kidney in rats has a peculiarity containing predominantly (more than 50%) highly active tonic neurons to electrical stimulation. In a previous publication (DOI: 10.1152/ajprenal.00011.2012) we demonstrated an increased mRNA expression of the TTX-resistant sodium channel Nav1.8 in renal sensory neurons. Hence, we tested the hypothesis that tonic firing pattern is related to the specific expression of Nav1.8 on the cell surface of neurons with renal sensoric axons in the dorsal root ganglia (DRG Th12-L2).
Methods
Harvested dorsal root ganglion neurons (DRG Th11-L2) from male Sprague Dawley (SD) with renal afferents were investigated in primary neuronal cell culture using current clamp mode to assess action potential generation during current injection and to characterize neurons as tonic highly active and phasic less active neurons using a Nav1.8 blocker (A-803467) before and after stimulation. Further, renal DRG neurons from a Nav1.8 knock out mouse (C57BL6J-Scn10atm1Jwo) were investigated in a current clamp mode. C57BL6 mice were used as controls.
Results
At a concentration of 0.3µM the maximum AP firing frequency was blocked from 13+/- 1.1 APs/600ms to 7.6+/-1.4 APs/600ms under superfusion with a Nav1.8 blocker. No blocker effects were seen at a concentration of 0.1 µM and due to superfusion with the solvent methanol alone.The firing pattern of renal neurons in the C57BL6 mouse was similar to that in the SD rat with a dominance of the tonic highly active neurons. In a Nav1.8 knock out mouse (C57BL6J-Scn10atm1Jwo) in the population of neurons with dendrites from the kidney only a single cell out of 70 showed tonic firing behavior (control vs Nav1.8 KO mouse, z-test, p<0.05).
Conclusion
Under physiological conditions, renal sensory neurons exhibit predominantly a firing pattern associated with higher excitability. Our findings in this study support the significance of the TTX-resistant sodium channel Nav1.8 for the specific tonic firing pattern of neurons with renal projections. That might be of importance for pharmacological interventions to influence renal nerve activity likely involved in the control of blood pressure and cardiovascular function.