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Abstract: SA-PO555

Uremic Toxins Are Adsorbed by the Model 3 Wearable Artificial Kidney (WAK)

Session Information

Category: Dialysis

  • 801 Dialysis: Hemodialysis and Frequent Dialysis

Authors

  • Gura, Victor, University of California Los Angeles David Geffen School of Medicine, Los Angeles, California, United States
  • Orantes, Carlos, Wearable Artificial Organs Inc., Beverly Hills, California, United States
  • Tabibian, Noble Benjamin, University of California Los Angeles David Geffen School of Medicine, Los Angeles, California, United States
  • ten Hoeve, Johanna, University of California Los Angeles David Geffen School of Medicine, Los Angeles, California, United States
Background

Elimination of uremic toxins is necessary for effective renal replacement therapy. Previous WAK data showed that its sorbents adsorb beta2 microglobulin, uric acid, hippuric acid, indoxyl sulphate and indoleacetic acid. We aimed to show the WAK3 effectively purifies dialysate from additional toxins.

Methods

Discarded dialysate from 8 dialysis patients was placed in a reservoir and recirculated for 4 hours through the the WAK3 charcoal cartridge. Samples were obtained before and after the fluid passage through the cartridge and analyzed LC-MS. Metabolites were separated on a LunaNH2 (Phenomenex) HILIC column and detected with a Q Exactive orbitrap mass spectrometer (Thermo Scientific). Statistical analysis was done with a one-way Anova test.

Results

the concentration of ADMA, homocitrulline, homocysteine, kynurenine, p-cresylsulfate, phenol, and phenylacetylglutamine, and methylguanidine in the spent dialysate decreased by 87.93% (p= <0.001) at a dialysate pulse flow rate of 30 ml/min.after 4 hours of recirculation through the WAK sorbent cartridge

Conclusion

The WAK3 effectively adsorbs uremic toxins from dialysate supporting the notion that the device may be effective in treating dialysis patients.

Percentage of toxin removed from dialysate after 4 hours of recirculation through the WAK3 charcoal sorbent
TOXIN% removedp-value
Methylguanidine74.4%<0.001
ADMA93.98%<0.001
Homocitrulline80.19%<0.001
Homocysteine72.33%<0.001
Kynurenine97.5%<0.001
p-Cresylsulfate96.71%<0.001
Phenol95.54%<0.001
Phenylacetylglutamine92.85%<0.001