ASN's Mission

To create a world without kidney diseases, the ASN Alliance for Kidney Health elevates care by educating and informing, driving breakthroughs and innovation, and advocating for policies that create transformative changes in kidney medicine throughout the world.

learn more

Contact ASN

1401 H St, NW, Ste 900, Washington, DC 20005

email@asn-online.org

202-640-4660

The Latest on X

Kidney Week

Abstract: SA-PO824

Proximal Tubular Sirt6 Has a Protective Role in the Murine Lupus Nephritis Model by Regulating Tubulointerstitial Inflammation

Session Information

Category: Glomerular Diseases

  • 1401 Glomerular Diseases: From Inflammation to Fibrosis

Authors

  • Wang, Tian, Jeonbuk National University Medical School, Jeonju, Jeollabuk-do , Korea (the Republic of)
  • Li, Wenjia, Jeonbuk National University Medical School, Jeonju, Jeollabuk-do , Korea (the Republic of)
  • Lee, Soo jin, Jeonbuk National University Medical School, Jeonju, Jeollabuk-do , Korea (the Republic of)
  • Shin, Yujin, Jeonbuk National University Medical School, Jeonju, Jeollabuk-do , Korea (the Republic of)
  • Kang, Kyung Pyo, Jeonbuk National University Medical School, Jeonju, Jeollabuk-do , Korea (the Republic of)
Background

Systemic lupus erythematosus (SLE), an autoimmune disease, involves tissue inflammation of multiple organs, including the kidney. Lupus nephritis is an autoimmune complex glomerulonephritis that develops as a complication of SLE. Dysregulation of intrarenal immune tolerance to nuclear autoantigens produces autoantibody and immune disorders. Sirt6 is the NAD+-dependent deacetylase and mono-ADP ribosyltransferase and is involved in genome maintenance and metabolism. This study investigates the effect of proximal tubule-specific Sirt6 knockdown on murine lupus nephritis.

Methods

To investigate the role of Sirt6 specifically in proximal renal tubules, we crossed gGt1-cre mice and Sirt6flox/flox mice to generate Sirt6 conditional knock-out mice. The back area's skin was shaved and treated topically three times per week, with 100 μg of resiquimod in 100 μl of acetone for eight weeks. After murine kidney sample collection, we evaluated renal histology and immunofluorescent study for inflammatory cells and lymphatic vessels. We also assessed inflammatory cytokines and chemokines, lymphangiogenic factors by qRT-PCR.

Results

The loss of Sirt6 in proximal tubules aggravates glomerular mesangial cell proliferation and tubulointerstitial inflammation, and there also increases glomerular deposition of IgG, IgM, and C3 in immunofluorescence staining. LYVE-1(+) lymphatic vessels have increased expression and infiltration of F4/80, CD11c, and B220 (+) inflammatory cells in proximal tubule-specific Sirt6 knock-out mice. The pro-inflammatory cytokines and chemokines such as ICAM-1, VCAM-1, MCP-1, BAFF, LTβ, and CXCL13 mRNA levels were increased compared with wild-type mice.

Conclusion

Our data suggest that proximal tubule Sirt6 is important in resiquimod-induced lupus nephritis, especially in regulating tubulointerstitial inflammation.

Funding

  • Government Support – Non-U.S.