Abstract: TH-PO945
Dietary Supplementation with Bacillus Superoxide Dismutase Protects Against Kidney Ischemia Reperfusion Injury in Mice
Session Information
- Health Maintenance, Nutrition, Metabolism - I
November 02, 2023 | Location: Exhibit Hall, Pennsylvania Convention Center
Abstract Time: 10:00 AM - 12:00 PM
Category: Health Maintenance, Nutrition, and Metabolism
- 1500 Health Maintenance, Nutrition, and Metabolism
Authors
- Yang, Jihyun, Kangbuk Samsung Hospital, Jongno-gu, Korea (the Republic of)
- Yoon Sook, Ko, Korea University Anam Hospital, Seoul, Korea (the Republic of)
- Hee Young, Lee, Korea University Anam Hospital, Seoul, Korea (the Republic of)
- Choi, Young Eun, Korea University Anam Hospital, Seoul, Korea (the Republic of)
- Chung, Suk Min, Korea University Anam Hospital, Seoul, Korea (the Republic of)
- Kim, Sungyeon, Korea University Anam Hospital, Seoul, Korea (the Republic of)
- Kim, Myung-Gyu, Korea University Anam Hospital, Seoul, Korea (the Republic of)
- Oh, Sewon, Korea University Anam Hospital, Seoul, Korea (the Republic of)
- Jo, Sang-Kyung, Korea University Anam Hospital, Seoul, Korea (the Republic of)
Background
Superoxide dismutase (SOD) is a metalloenzyme that play an important role in antioxidant defense against oxidative stress. Bacillus strains are known to produce manganese containing SOD (Mn-SOD) and thus have been shown to have protective effect in inflammatory bowel disease animal model. Based on recent data showing the important role of gut dysbiosis, aberrant gut mucosal immunity as well as well-known oxidative stress in kidney injury, we investigated the renoprotective potential of orally administered enteric-coated SOD protein purified from Bacillus amyloliquefaciens (SOD-BA) in kidney ischemia/reperfusion injury (IRI) in mice.
Methods
Six week old C57BL/6 male mice were fed with SOD-BA 24 hours prior to IRI. Serum BUN, creatinine and histologic alterations as well as oxidative stress, inflammation in kidney tissue were determined. Gut microbiome analysis, permeability, epithelial damage as well as various antioxidant enzymes including catalase and SOD activity were also determined.
Results
Oral supplementation of SOD-BA conferred significant renoprotective effect in kidney IRI and this was associated with significantly increased level of SOD levels in kidneys. Gut microbiota structure in SOD-BA +IRI group was clearly distinguished from that of IRI group in principal coordinate analysis, showing that the relative frequency of family Porphyromonadaceae, Muribaculaceae and Bacteroidacea increased. SOD-BA administration also led to decreased colon epithelial apoptosis with partial restoration of gut permeability. Despite significant renoprotective effect, there was no difference in colon and kidney inflammation.
Conclusion
SOD-BA could be served as an orally available, potent antioxidant in the prevention and treatment of acute kidney injury. Our data showed a therapeutic potential of gut bacterial product in kidney IRI that can overcome the safety issue regarding the use of probiotics.