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Abstract: FR-PO729

GADD45A and GADD45B as Novel Biomarkers Associated with Chromatin Regulators in Renal Ischemia-Reperfusion Injury and Their Correlation with Immune Cells

Session Information

  • Transplantation: Basic
    November 03, 2023 | Location: Exhibit Hall, Pennsylvania Convention Center
    Abstract Time: 10:00 AM - 12:00 PM

Category: Transplantation

  • 2101 Transplantation: Basic

Authors

  • Xie, Ming, Southern Medical University Nanfang Hospital, Guangzhou, Guangdong, China
  • Xie, Ruiyan, Queen Mary Hospital, Hong Kong, Hong Kong
  • Yap, Yat Hin Desmond, Queen Mary Hospital, Hong Kong, Hong Kong
  • Wu, Peng, Southern Medical University Nanfang Hospital, Guangzhou, Guangdong, China
Background

The role of chromatin regulators (CRs) in the pathogenesis of renal ischemia-reperfusion injury (IRI) remains unclear.

Methods

We performed a bioinformatic analysis on the differentially expressed genes of CRs in renal IRI patients using machine learning methods. The hub CRs identified were used to develop a risk prediction model for renal IRI, and their expressions were validated using a murine renal IRI model. We also examined the relationships between hub CRs and infiltrating immune cells in renal IRI.

Results

GADD45A and GADD45B were upregulated in renal IRI. GADD45A and GADD45B were chosen to develop a renal IRI risk prediction model and showed good performance. GADD45A and GADD45B showed correlations with plasmacytoid dendritic cells (pDCs) in infiltrating immune cells analysis.

Conclusion

Dysregulation of GADD45A and GADD45B is related to renal IRI and the infiltration of pDCs.

Identification of CRs-associated differentially expressed genes in renal ischemia-reperfusion injury using machine learning models, construction and validation of a CRs-associated predictive nomogram.

Correlation analysis of GADD45A and GADD45B expression with cell surface markers of pDCs in renal IRI.

Funding

  • Government Support – Non-U.S.