Cell Volume-Sensitive Transcription Factor(s) Regulate Claudin-2 Expression in Epithelial Cells
- Fluid, Electrolyte, Acid-Base Disorders: Basic
November 03, 2023 | Location: Exhibit Hall, Pennsylvania Convention Center
Abstract Time: 10:00 AM - 12:00 PM
Category: Fluid, Electrolytes, and Acid-Base Disorders
- 1101 Fluid, Electrolyte, and Acid-Base Disorders: Basic
- Koumangoye, Rainelli, Vanderbilt University Medical Center, Nashville, Tennessee, United States
- Delpire, Eric J., Vanderbilt University Medical Center, Nashville, Tennessee, United States
The basolateral Na+-K+-2Cl- cotransporter (NKCC1) plays a critical role in maintaining the intracellular concentrations of Na+, K+, and Cl- ions, and cell volume. While it is well recognized that NKCC1 is critically important for vectorial transport of Na+, K+, and Cl- across epithelia, its role in transcellular ions transport and epithelial barrier function is not well understood. Here we ask whether loss of NKCC1 function affect kidney and intestinal epithelial tight junction permeability.
Wild-type MDCK I and NKCCC1-deficient clones LKA3 and LKC1 were plated and polarized on transwell filters, and their TER was measured. Tight junction protein expression and localization was assessed by western blotting and immunofluorescence.
The TER values of NKCC1-deficient MDCK I cells decreased by more than 20-fold, from 8000 Ω.cm2 in parental MDCK I to 300-500 Ω.cm2 in LKA3 and LKC1 clones. Western blot analysis showed that genetic ablation or pharmacologic of NKCC1 function increases the expression of the cation selective claudin-2. This is consistent with the observed decreased TER. In addition, growing MDCK I cells in Cl--free, Na+-free or both Cl- and Na+-free media upregulates claudin-2 expression.
Our data indicate that loss of NKCC1 function affects tight junction protein expression without affecting their localization. Particularly, claudin-2 was significantly upregulated. This led to an increase in paracellular flux of positively charged ions across the MDCK epithelial cell monolayers. Notably, this phenomenon is not unique to kidney epithelial cells cells, as loss of NKCC1 function in intestinal HT29 cells also led to significant decrease in TER and upregulation of claudin-2 expression.
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