ASN's Mission

To create a world without kidney diseases, the ASN Alliance for Kidney Health elevates care by educating and informing, driving breakthroughs and innovation, and advocating for policies that create transformative changes in kidney medicine throughout the world.

learn more

Contact ASN

1401 H St, NW, Ste 900, Washington, DC 20005

email@asn-online.org

202-640-4660

The Latest on X

Kidney Week

Abstract: FR-PO1095

Higher Serum IL-6 and IFN-γ Are Associated with Increased Seroconversion Rate Against SARS-CoV-2 Spike Protein Either After Vaccination or SARS-CoV-2 Infection

Session Information

  • COVID-19 - II
    November 03, 2023 | Location: Exhibit Hall, Pennsylvania Convention Center
    Abstract Time: 10:00 AM - 12:00 PM

Category: Coronavirus (COVID-19)

  • 000 Coronavirus (COVID-19)

Authors

  • Prasad, Narayan, Sanjay Gandhi Post Graduate Institute of Medical Sciences, Lucknow, Uttar Pradesh, India
  • Yadav, Brijesh, Sanjay Gandhi Post Graduate Institute of Medical Sciences, Lucknow, Uttar Pradesh, India
  • Gautam, Sonam, Sanjay Gandhi Post Graduate Institute of Medical Sciences, Lucknow, Uttar Pradesh, India
  • Yadav, Deependra Kumar, Sanjay Gandhi Post Graduate Institute of Medical Sciences, Lucknow, Uttar Pradesh, India
Background

Maintenance immunosuppressives lead to a decreased immunogenic response to SARS-CoV-2 infection and vaccination in renal transplant recipients (RTRs) than in healthy populations. However, healthy people with SARS-CoV-2 infection often develop neutralizing antibodies and secrete high quantities of cytokines leading to virus clearance and sometimes more severe COVID-19. Associations of cytokines with seroconversion rate in immunocompromised RTRs are not well studied, particularly in living donor scenarios.

Methods

In this study, we included 210 living-related RTRs, who acquired SARS-CoV-2 infection or were vaccinated with two doses of either COVISHIELD or COVAXIN; and 35 healthy controls who were neither vaccinated nor had anti-SARS-CoV-2 spike IgG as controls. Cytokines IL-6, IFN-γ, TGF-β, and IL-10 were measured using the ELISA, and SARS-CoV-2 spike protein-specific IgG titre by chemiluminescent microparticle immunoassay methods.

Results

We found a seroconversion rate of 115/132 (87.12%), with a median antibody titer of 706.40 au/ml (IQR, 215.45-1844.42) in the infection group and 63/78 (80.76%) with a median titer 1454.20 (IQR, 80.52-3838.75) au/ml in vaccination group. The IL-6, IFN-γ, TGF-β, and IL-10 levels were significantly higher in both the infection and vaccination groups than in the healthy control. In the infection group, IL-6 (55.41±24.30 vs 31.64±16.98 pg/ml; p<0.001); IFN-γ (91.21±33.09 vs 61.69±33.28 pg/ml; p=0.001), were significantly higher in the seroconverter group as compared to non-seroconverter. TGF-β (730.48±400.47 vs 765.47±366.39 pg/ml; p=0.92) and IL-10 (91.31±48.54 vs 96.73±59.53 pg/ml; p=0.88) were similar between seroconverter and non-seroconverter. Similarly, in the vaccination group, IL-6 (50.31±25.67 vs 30.00±11.19 pg/ml; p=0.002) and IFN-γ (65.70±39.78 vs 32.14±17.48 pg/ml; p=0.001) were significantly higher in seroconverter post-vaccination compared to non-seroconverter. In contrast, TGF-β (820.96±415.78 vs 1045.57±204.66; p=0.046) was elevated in non-seroconverter, and although IL-10 (93.18±35.45 vs 112.90±59.61 pg/ml; p=0.11) was similar between seroconverter and non-converter.

Conclusion

Inflammatory cytokines IL-6 and IFN-γ are significantly associated with higher seroconversion rates in RTRs after SARS-CoV-2 infection and vaccination.